ABSTRACT
A new biosensor was prepared based on conductive polymer poly ( 3 , 4-ethylenedioxythiophene ) ( PEDOT) and 1-pyrenebutanoic acid ( PBA) through π-π stacking, as well as hematin associated with PBA through coordinate bonds of Zr4+ and carboxyl group. Its stability and sensitivity were examined by cyclic voltammetry ( CV) , electrochemical impedance spectroscopy ( EIS) and current-time ( i-t) method. A pair of well defined and quasi-reversible redox peaks was observed when GCE/PEDOT/PBA/hematin was tested by CV in PBS without oxygen. The electron transfer rate constant was estimated to be 4. 8 s-1 . Results indicated that the PEDOT film enhanced the electron transfer process of hematin. When the i-t method was used to detect the response of biosensor to catechol with different concentrations, it displayed a linear response for the reduction of catechol in the range of 0. 5-200 μmol/L. The linear equation was i=0. 018C+0. 006 ( R=0. 9998), and the detection sensitivity was 0. 258 μA (μmol/L· cm2) with a detection limit of 0. 33 nmol/L (S/N=3). The results illustrate that the GCE/PEDOT/PBA/hematin biosensor is very sensitive and stable.
ABSTRACT
Objective To investigate the role of sex chaperone on self-acceptance,anxiety,depression and coping strategy in stable phase extensive burn patients'.Methods According to the different role of accompanies,the extensive burn patients in stable phase were divided into sex chaperone accompany groups(treatment group)and non-sex chaperone accompany group(control group).They were assessed by self-acceptance questionnaire(SAQ),self-rating anxiety scale(SAS),self-rating depression scale(SDS)and medical coping modes questionnaire(MCMQ).The results were statistically analyzed.Results The treatment group patients'results are as follows:SAQ total score was(41.37±6.98),self-acceptance factor score was(20.56±4.87),self-evaluation factor score was(22.35±5.82),MCMQ facing factor score was(21.80±4.07),MCMQ avoiding factor score was(22.35±5.82).The results of treatment group patients were significantly higher than those in control group,it had significant differences(P<0.05 or P<0.01);while the treatment group patients'scores of SAS,SDS,MCMQ yield factor were significantly lower that those in the contorl group.The self-acceptance factor score appeared to be negative correlation with the SAS score,SDS score and MCMQ yield factor score,while to be positive correlation with MCMQ facing/avoiding factor score.Conclusion Sex chaperone'accompany can eliminate the extensive burn patients'tension and the effect of adverse psychological factors,and meet family love needs of patients,then improve the level of patient self-acceptance,reverse the negative emotion of patients,and guide patients in a positive way to face the disease.